Hi, this confuses me long time. How did 454 machine tell whether it is a A, T, C or G incorporated?
APS+PPi ATP sulfurylase
ATP + Luciferin Light + Oxyluciferin luciferase
There is always one PPi, if add one nt into the DNA strand. Not matter a A, T, C , G is added? How does the machine tell the light strength is from adding an A or C.
thanks
APS+PPi ATP sulfurylase
ATP + Luciferin Light + Oxyluciferin luciferase
There is always one PPi, if add one nt into the DNA strand. Not matter a A, T, C , G is added? How does the machine tell the light strength is from adding an A or C.
thanks
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