hi, everyone. To find mutation information between two bacteria, especially SNPs, we sequenced mutated strain by SOLEXA. I assembled the genome and compared with genome of WT, got some SNPs. in additional, I use novoalign and MAQ to align short reads to reference and got some SNPs. but the results from compare between genomes are not included in align results. because alignment always gave results including more negative SNPs, and I believe the SNPs through compare genome are more accurate. thanks.
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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