hi, everyone. To find mutation information between two bacteria, especially SNPs, we sequenced mutated strain by SOLEXA. I assembled the genome and compared with genome of WT, got some SNPs. in additional, I use novoalign and MAQ to align short reads to reference and got some SNPs. but the results from compare between genomes are not included in align results. because alignment always gave results including more negative SNPs, and I believe the SNPs through compare genome are more accurate. thanks.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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