hi, everyone. To find mutation information between two bacteria, especially SNPs, we sequenced mutated strain by SOLEXA. I assembled the genome and compared with genome of WT, got some SNPs. in additional, I use novoalign and MAQ to align short reads to reference and got some SNPs. but the results from compare between genomes are not included in align results. because alignment always gave results including more negative SNPs, and I believe the SNPs through compare genome are more accurate. thanks.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM