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  • cmccabe
    Senior Member
    • Jul 2012
    • 355

    #1

    add missing annotations to vcf

    I am using bcftools version 1.3.1 and trying to add undefined annotations to a vcf before using bgzip and tabix.

    If I try to run the norm command on the vcf I get:
    Code:
    bcftools norm -m-both -o file.vcf file.vcf.gz
         
    [W::vcf_parse] INFO 'RSC1A1,RSC1A1' is not defined in the header, assuming Type=String
         Could not parse the header line: "##INFO=     <ID=RSC1A1,RSC1A1,Number=1,Type=String,Description="Dummy">"
    So I use:
    Code:
    # find undefined annotations#
    bcftools view -h file.vcf > header.txt
    # add undefined to vcf#
    bcftools reheader -h header.txt file.vcf > fixed.vcf
    I then use bgzip and tabix on the fixed.vcf and rerun the norm command:
    Code:
     bcftools norm -m-both -o fix.vcf fixed.vcf.gz
         [W::vcf_parse] INFO 'RSC1A1,RSC1A1' is not defined in the header, assuming Type=String
         Could not parse the header line: "##INFO= <ID=RSC1A1,RSC1A1,Number=1,Type=String,Description="Dummy">"
         [E::vcf_parse] Could not add dummy header for INFO 'RSC1A1,RSC1A1'
         [W::vcf_parse] INFO 'RSC1A1,RSC1A1' is not defined in the header, assuming Type=String
         Could not parse the header line: "##INFO=<ID=RSC1A1,RSC1A1,Number=1,Type=String,Description="Dummy">"
         [E::vcf_parse] Could not add dummy header for INFO 'RSC1A1,RSC1A1'
         Lines   total/split/realigned/skipped:	116/4/0/0
    It seems the dummy line with the undefined annotation was not added to the vcf, but I am not sure how to fix it. Thank you .
    Last edited by cmccabe; 03-01-2017, 04:55 AM.
  • finswimmer
    Member
    • Oct 2016
    • 60

    #2
    Hello,

    is a comma really allowed in the ID definition? Maybe with quotes around?

    fin swimmer

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