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  • Palgrave
    Member
    • Aug 2011
    • 73

    fastx_collapser: found invalid nucleotide sequence

    Anyone know what the reason is?

    fastx_collapser: found invalid nucleotide sequence (TCTTAACCCGGACCAGAAACTA ) on line 2




    >1-1460323
    TCTTAACCCGGACCAGAAACTA
    >2-1321361
    AGGTTCCGGATAAGTAAGAGCC
    >5-342436
    TAGCATATCGAGCCTGAGAACA
    >6-267424
    TGATACGGATGTTATACGCAGC
    >7-221604
    CGGTTCCGGATAAGTAAGAGCC
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Not answering your question but wanted to suggest that you may want to try clumpify.sh from BBMap for this purpose (http://seqanswers.com/forums/showpos...3&postcount=29). See full thread for additional information.

    Comment

    • gringer
      David Eccles (gringer)
      • May 2011
      • 845

      #3
      It could be the space at the end of the line. I notice that there is no space after the opening bracket, and one space before the closing bracket.

      Comment

      • Palgrave
        Member
        • Aug 2011
        • 73

        #4
        I think so. Any idea how to remove it?

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          Code:
          sed 's/\ $//g' your_file > new_file
          should do it.

          Comment

          • Palgrave
            Member
            • Aug 2011
            • 73

            #6
            Thanks. It worked

            Comment

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