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coverage by strand with pysam

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  • adamdeluca
    replied
    BEDTools's genomeCoverageBed will calculate strand-specific coverage.

    http://code.google.com/p/bedtools/

    Leave a comment:


  • desaila
    started a topic coverage by strand with pysam

    coverage by strand with pysam

    I'm pretty new to NGS analysis (and this forum), and have been mapping my data with bfast. I'm interested in coverage by strand, and am trying to come up with the best way to do this.

    Looking through the pysam documentation
    Code:
    import pysam
    samfile = pysam.Samfile("ex1.bam", "rb" )
    for pileupcolumn in samfile.pileup( 'chr1', 100, 120):
        print
        print 'coverage at base %s = %s' % (pileupcolumn.pos , pileupcolumn.n)
        for pileupread in pileupcolumn.pileups:
            print '\tbase in read %s = %s' % (pileupread.alignment.qname,
            pileupread.alignment.seq[pileupread.qpos])
    Iterating over the bam file this way seems ideal, but Is there a way to pull out the +/- strand info from a pilupcolumn object? Or, would is doing something like this more appropriate?

    Code:
     
    for pileupcolumn in samfile.pileup( 'chr1', 100, 120):
        for pileupread in pileupcolumn.pileups:
            if pileupread.alignment.is_reverse: #negative strand hit
                negCov[ pileupcolumn.pos ] += 1
            else:
                posCov[ pileupcolumn.pos ] += 1
    My primary concern is correctness, but a secondary concern is run time. This nested for loop seems costly, is that a good assumption?
    Last edited by desaila; 10-14-2010, 08:09 AM.

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