Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • elaJS
    Junior Member
    • Apr 2017
    • 1

    #1

    cuffmerge errors

    Hi,

    While running cuffmerge I am facing the following error:
    Traceback (most recent call last):
    File "/software/cufflinks-2.2.1/cuffmerge", line 580, in ?
    sys.exit(main())
    File "/software/cufflinks-2.2.1/cuffmerge", line 554, in main
    sam_input_files = convert_gtf_to_sam(gtf_input_files)
    File "/software/cufflinks-2.2.1/cuffmerge", line 287, in convert_gtf_to_sam
    sam_out = gtf_to_sam(line)
    File "/software/cufflinks-2.2.1/cuffmerge", line 252, in gtf_to_sam
    exit(1)
    TypeError: 'str' object is not callable


    I used the following command:
    bsub -m tarrcwn4 -R"rusage[mem=3000]" -o merge_time1.log.out '/software/cufflinks-2.2.1/cuffmerge -p 1 -o MERGED -g /shared/live/000_rawData/001_HBdata/Hbgenome.gtf -s /shared/live/001_rootTranscripts/004_RNAseq/hb_genome.fa assembly_gtf.txt'

    I must say I have done mapping with STAR and then use samtools, cufflinks to generate transcripts.gtf files. The paths to transcripts.gtf files are in assembly_gtf.txt

    Thanks in advance for your help

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, Yesterday, 11:13 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, Yesterday, 10:48 AM
0 responses
18 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
36 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
42 views
0 reactions
Last Post SEQadmin2  
Working...