Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • naono
    Junior Member
    • Dec 2012
    • 5

    #1

    Trinity freezes on clustering

    Recently we tried to replicate our previous result of de novo assembly using Trinity 2.0.6.
    and
    Bowtie 1.2.0

    First it seems working but Just after
    "Trinity Phase 1: Clustering of RNA-Seq Reads",
    on the command

    cmd: bash -c " set -o pipefail; bowtie -a -m 20 --best --strata --threads 32 --chunkmbs 512 -q -S -f \
    ./chrysalis/inchworm.K25.L25.fa.min100 both.fa | samtools view -@ 32 -F4 -Sb - | samtools sort -@ 32 -no - - > /project/hikaku_db/nono/sync/opium_poppy2/trinity.3s1/trinity.POL2/chrysalis/iworm.bowtie.nameSorted.bam" 2>/dev/null
    1
    it seems to freeze for hours and days without any output.

    Also, I run the corresponding command directly, instead of use pipeline, save the output for a temporary file, but the process froze again before finishing to write the temporal output file.

    Does anyone has some comment or suggestion to solve the problem ?
  • naono
    Junior Member
    • Dec 2012
    • 5

    #2
    I have edited the cmd to use bowtie version 1.0.0, and it worked.

    Can someone guess what was the problem?

    Comment

    • naono
      Junior Member
      • Dec 2012
      • 5

      #3
      The same trouble occurred using
      Trinity 2.4.0
      Bowtie 2.3.1
      Samtools 1.4

      It goes to the line

      * Running CMD: bash -c " set -o pipefail;bowtie2 --local -k 2 --threads 16 -f --score-min G,46,0 \
      -x chrysalis/inchworm.K25.L25.fa.min100 both.fa | samtools view - @ 16 -F4 -Sb - | samtools \
      sort -m 4294967296 -@ 16 -no - - > chrysalis/iworm.bowtie.nameSorted.bam"

      and stalled without creating output file.
      Last edited by naono; 05-12-2017, 04:29 PM.

      Comment

      • naono
        Junior Member
        • Dec 2012
        • 5

        #4
        I updated samtools to 1.4.1 and it worked!!

        Trinity 2.4.0
        Bowtie 2.3.2
        Samtools 1.4.1

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
          by SEQadmin2




          NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
          Yesterday, 06:46 AM
        • SEQadmin2
          Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
          by SEQadmin2



          Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

          Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
          ...
          10-14-2026, 09:55 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, 10-28-2026, 09:57 AM
        0 responses
        27 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-28-2026, 09:51 AM
        0 responses
        18 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 11:13 AM
        0 responses
        71 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 10-22-2026, 10:48 AM
        0 responses
        48 views
        0 reactions
        Last Post SEQadmin2  
        Working...