Baits in WGS???

[Joseph White]

Hi,

Capture baits are used for exome sequencing in WES. Are baits used for whole genome sequencing. Or, is sequencing randomly primed for WGS?

Thanks for any info.
Cheers,
jwhite

[nucacidhunter]

Common method for WGS library prep is ligating adapters to randomly sheared DNA. There are also less common methods such as tagmentation that involves less randomly cutting DNA and inserting a sequence overhang for subsequent PCR amplification. Although technically incorrect, you can think of these methods as randomly primed sequencing.