Hi,
I am struggling to find possible explanations for seeing two distinct peaks in my "per sequence quality scores". I am very new to this, so hope it's not a very silly question. As far as I understand it should not be a major concern, however I would like to understand why this is.
There is also two peaks for GC content in sequences, with very low adapter content at the end of reads.
Sanger/Illumina 1.9 encoding
The sequence data is from an unknown clinical sample from hospital, for the purpose of identifying possible pathogens.
Kind regards,
Wilro
I am struggling to find possible explanations for seeing two distinct peaks in my "per sequence quality scores". I am very new to this, so hope it's not a very silly question. As far as I understand it should not be a major concern, however I would like to understand why this is.
There is also two peaks for GC content in sequences, with very low adapter content at the end of reads.
Sanger/Illumina 1.9 encoding
The sequence data is from an unknown clinical sample from hospital, for the purpose of identifying possible pathogens.
Kind regards,
Wilro
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