Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • MGCBrown
    Member
    • May 2017
    • 15

    undescribed snpEff error after command execution

    Hello,

    I'm trying to use snpEff to annotate variants in a .vcf file that I called with SAMtools.

    Here is my command:

    java -Xmx4g -jar snpEff.jar -v spetri2.86 glaucomysvarfiltered.vcf > glaucomysvarfiltered.annotated.vcf

    After it loads the database and prints "building interval forest", it starts rapidly outputting a bunch of symbols in my terminal window, and when I check to see if I have any process running via $ top, there is no snpEff processes running, and it discontinues printing to my output file.

    I tried it a few times now, and the random symbols have continued to be printed on the screen for over an hour.

    I've attached a screen shot of the last processes before it goes haywire, and a screen shot of the symbols.
    Attached Files
  • MGCBrown
    Member
    • May 2017
    • 15

    #2
    I've noticed that what I thought was a .vcf file created with the SAMtools variant calling pipeline is actually a vCalendar file.

    Any explanations as to why I have a vCalendar file? I'm guessing it is not the same as a variant call format (VCF) file.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    • SEQadmin2
      From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
      by SEQadmin2


      Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


      The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
      ...
      06-02-2026, 10:05 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Today, 05:37 AM
    0 responses
    5 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    16 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    50 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-09-2026, 11:58 AM
    0 responses
    110 views
    0 reactions
    Last Post SEQadmin2  
    Working...