Hi,
I am working on elucidating pathologically relevant miRNAs responsible for disease progression and chemoresistance in chronic lymphocytic leukemia. For differential expression of miRNAs I am using the edgeR package. I am new to statistics, edger and any help would be highly appreciated. The samples are classified as 1) Indolent which has 7 patient samples 2) Chemo-responsive, 9 patient samples 3) Non-responsive, 7 patient samples 4) Control, 2 samples.
I want to check the differential expression of miRNAs as follows:
For comparing two groups like indolent vs chemo-responsive contrast matrix contrast(-1,1,0,0) works well. What should be the contrast matrix when comparing:
(a) Control vs Indolent,Chemo-responsive,Non-responsive
(b) Indolent vs Chemo-responsive,Non-responsive.
-
The COVID-19 pandemic highlighted the need for proactive pathogen surveillance systems. As ongoing threats like avian influenza and newly emerging infections continue to pose risks, researchers are working to improve how quickly and accurately pathogens can be identified and tracked. In a recent SEQanswers webinar, two experts discussed how next-generation sequencing (NGS) and machine learning are shaping efforts to monitor viral variation and trace the origins of infectious...03-24-2025, 11:48 AM -
This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.
The Headliner
The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...03-03-2025, 01:39 PM