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  • Kamy24
    started a topic Differential expression of miRNAs using edgeR

    Differential expression of miRNAs using edgeR

    Hi,
    I am working on elucidating pathologically relevant miRNAs responsible for disease progression and chemoresistance in chronic lymphocytic leukemia. For differential expression of miRNAs I am using the edgeR package. I am new to statistics, edger and any help would be highly appreciated. The samples are classified as 1) Indolent which has 7 patient samples 2) Chemo-responsive, 9 patient samples 3) Non-responsive, 7 patient samples 4) Control, 2 samples.
    I want to check the differential expression of miRNAs as follows:

    For comparing two groups like indolent vs chemo-responsive contrast matrix contrast(-1,1,0,0) works well. What should be the contrast matrix when comparing:

    (a) Control vs Indolent,Chemo-responsive,Non-responsive
    (b) Indolent vs Chemo-responsive,Non-responsive.

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM

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