Originally posted by mido1951
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Oh - I see the confusion. BBMap is not capable of mapping unlimited-length reads. It can only map reads up to 600bp. mapPacBio.sh is a version of BBMap that supports longer reads, up to 6000bp. Reads longer than that will be shredded into shorter pieces which are mapped and tracked individually. So it's not ideal for long read platforms for Nanopore when you need full-length alignments.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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