Detection of Fusion Gene Events from RNASeq PE reads

[zee]

Hi Everybody,

I am looking for the best strategy for finding gene fusion events in some human resequencing RNASeq paired-end reads.
At present there is one package I am aware of called DeFuse, however I think that tools such as BreakDancer, Breakway and Hydra-SV would be suitable for this task with some downstream operations.

I have my reads mapped to HG18 with novoalign in BAM format. Some pointers from people with experience would be great.

Thanks

[krobison]

You might also check out Trans-ABySS and FusionSeq

I haven't (yet) gotten experience with any of these.

[kissthefuture]

I think you can look this page :http://compbio.bccrc.ca/?page_id=600

there is a workflow for you