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  • nurgling
    Junior Member
    • Jan 2017
    • 3

    #1

    Readseq identifying the wrong DATATYPE

    Hi there,

    Having problems with readseq.jar and not finding an answer anywhere.

    Input:
    Amino acid .fasta alignments, truncated example:
    >ALEXANDRIUM-CATENELLA_TRINITY_DN55193_C0_G1_I1_5
    --SVLEQLRSSHEDIENIEKAMS-------K.......SS.......KQT.......VSCEHALKYLIE---N...K...S...KT...A...VEIYQDKDGMRTDDINAL--------Q........GDV
    >DINOPHYSIS-ACUMINATA_TRINITY_DN39905_C0_G3_I1_2
    ---SVLEQLRSAHEDIENIEKAMS-------K.......ST.......RNT.......VACEHSLKYLID...T---K...C...KT...A...VDIYQDKDGMRSDDINAL---

    command: Turn .fasta into a .nexus file
    `java -jar /PATHs/readseq.jar -f17 EPrGT00050000000311.clean.aln.fa`

    `head` of output .nexus file:
    #NEXUS
    BEGIN DATA;
    DIMENSIONS NTAX=23 NCHAR=1007;
    FORMAT DATATYPE=DNA INTERLEAVE MISSING=-;
    [Name: ALEXANDRIUM-CATENELLA_TRINITY_DN55193_C0_G1_I1_5 Len: 1007 Check: 0]
    [Name: DINOPHYSIS-ACUMINATA_TRINITY_DN39905_C0_G3_I1_2 Len: 1007 Check: 0]

    Problem: DATATYPE=DNA which should be PROTEIN instead. Previously it would designate the correct datatype.
    Problem is occuring on both laptop and cluster.

    Questions:
    Has this happened to anyone else?
    Any idea what may be confusing readseq as to the DATATYPE?
    Is it possible to force readseq to set the input as AAs?

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