Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • finswimmer
    Member
    • Oct 2016
    • 60

    #1

    Questions concerning bbmerge

    Hello all,
    I have some question concerning bbmerge. I would like to merge overlapping read pairs. For this I use this command:

    Code:
    bbmerge-auto.sh in1=Sample_L001_R1_001.fastq.gz in2=Sample_L001_R2_001.fastq.gz out=merged/Sample.extendedFrags.fastq.gz outu1=merged/Sample.notCombined_1.fastq.gz outu2=merged/Sample.notCombined_2.fastq.gz qtrim2 trimq=10,20,30 ordered=t ftm=5
    When having multiple values for trimq I get the following error

    Code:
    Exception in thread "Thread-6" java.lang.NullPointerException
    	at jgi.BBMerge$MateThread.processReadPair(BBMerge.java:2341)
    	at jgi.BBMerge$MateThread.findOverlapInThread(BBMerge.java:1643)
    	at jgi.BBMerge$MateThread.processReads(BBMerge.java:1583)
    	at jgi.BBMerge$MateThread.run(BBMerge.java:1557)
    Output buffer became full; key 1250 waiting on 995.
    And the program stop. If I remove the ordered=t option this appears:

    Code:
    Exception in thread "Thread-7" java.lang.NullPointerException
    	at jgi.BBMerge$MateThread.processReadPair(BBMerge.java:2341)
    	at jgi.BBMerge$MateThread.findOverlapInThread(BBMerge.java:1643)
    	at jgi.BBMerge$MateThread.processReads(BBMerge.java:1583)
    	at jgi.BBMerge$MateThread.run(BBMerge.java:1557)
    Exception in thread "Thread-9" java.lang.NullPointerException
    	at jgi.BBMerge$MateThread.processReadPair(BBMerge.java:2341)
    	at jgi.BBMerge$MateThread.findOverlapInThread(BBMerge.java:1643)
    	at jgi.BBMerge$MateThread.processReads(BBMerge.java:1583)
    	at jgi.BBMerge$MateThread.run(BBMerge.java:1557)
    Exception in thread "Thread-6" java.lang.NullPointerException
    	at jgi.BBMerge$MateThread.processReadPair(BBMerge.java:2341)
    	at jgi.BBMerge$MateThread.findOverlapInThread(BBMerge.java:1643)
    	at jgi.BBMerge$MateThread.processReads(BBMerge.java:1583)
    	at jgi.BBMerge$MateThread.run(BBMerge.java:1557)
    But the program runs fine until it's finished.

    What causes this trouble and can I fix it?

    Another question is: Can I do quality trimming for reads that do not overlap directly with in this step?

    fin swimmer
  • finswimmer
    Member
    • Oct 2016
    • 60

    #2
    Ok, I could fix the error messages by setting qtrim2 to one of the values mentioned in the manual for qtrim.

    Code:
    qtrim=f              Trim read ends to remove bases with quality below minq.
                         Trims BEFORE merging.
                         Values: t (trim both ends), 
                                 f (neither end), 
                                 r (right end only), 
                                 l (left end only).
    qtrim2=f             May be specified instead of qtrim to perform trimming 
                         only if merging is unsuccessful, then retry merging.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...