Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Splinter479
    Junior Member
    • Aug 2012
    • 6

    #1

    Reconstructing a de Bruijn Graph from Velvet's LastGraph

    Hi,

    I was wondering if someone can explain/ give hints to me about how to regain a de Bruijn Graph from Velvet's LastGraph representation of nodes and arcs?

    I created an example with hashsize (k)=7 and told velvetg to "keep all data" by setting cov_cutoff=1 min_contig_lgth=1:

    Code:
    >SEQUENCE_0
    TTTTTTCATGCATGCTAGCGTGTGTGTGT
    >SEQUENCE_1
    GTGTGTGTGTTAGCTGCAGTATGCGGAAC
    >SEQUENCE_2
    ACACACACACATCGATTGTTATGCGGAAC
    >SEQUENCE_3
    TATGCGGAACGCATTGCTAACTCGGGGGG
    results in

    Code:
    5	4	7	1
    NODE	1	12	13	13	0	0
    GCTAGCATGCAT
    GCTAGCGTGTGT
    NODE	2	1	6	6	0	0
    G
    C
    NODE	3	1	7	7	0	0
    T
    A
    NODE	4	15	15	15	0	0
    TAGCTGCAGTATGCG
    CTGCAGCTAACACAC
    NODE	5	14	14	14	0	0
    TCGATTGTTATGCG
    ACAATCGATGTGTG
    ARC	1	-1	2
    ARC	-1	2	1
    ARC	2	3	6
    ARC	-2	-3	4
    ARC	3	4	1
    ARC	-3	5	1
    I don't get exactly HOW to read out the sequence from the traversal of the nodes, e.g. (1) --> (-1), (-1) --> (2) and so on.
    Once I know how to read the sequence I could rebuild a simple de Bruijn Graph from it. Or is there maybe some simpler (back-)transformation from LastGraph to de Bruijn Graph?

    The (very old) manual page of Velvet is not helping too much

    Any help highly appreciated.

    Thanks!


    ------------------------------
    EDIT
    ------------------------------

    Another example:
    Let's take a sequence s=ACTGGACTGAA
    As I recall this results in the dBG of s:
    Code:
    ACT --> CTG --> TGG --> GGA --> GAC --> (ACT...)
             | 
             |--> TGA --> GAA
    Velvet's result is (k=3, velvetg cov_cutoff=1 min_contig_lgth=1):
    1 1 3 1
    NODE 1 5 7 7 0 0
    TGGAC
    CCAGT
    ARC 1 1 1

    This means I would be able to recover:
    Code:
    (Node 1 upper seq.)              TGGAC
                                     |||    --> ACTGGAC  (and an arbitrary amount of concatenations of this seq. since ARC (1) --> (1) )
    (Node 1 lower seq. rev.comp.)  ACTGG
    BUT, it seems to me that Velvet is missing the entire alternative path "TGAA" of the dBG of s.
    I would have expected another node and an additional arc to find that sequence.

    Am I right until here? Do I miss some/ should I change some parameter?
    Last edited by Splinter479; 07-12-2017, 11:26 PM.

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
23 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
29 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-13-2026, 09:56 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-09-2026, 11:14 AM
0 responses
28 views
0 reactions
Last Post SEQadmin2  
Working...