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  • lagrace
    Junior Member
    • May 2017
    • 4

    #1

    NA's in data (using TopHat-HTSeq-DESeq)

    Hello all,

    I have some data that I have retrieved from GEO. I am using the TopHat-HTSeq-DESeq pipeline to analyze this data. Within linux, I am using R and ran into an issue with NA's in the data. I am trying to determine why there are so many NA's. I was told to view the annotations. Is that possible in R? If so, how?
    Not sure if there is some other reason. Below are the codes used:

    samTab=read.table(file="samTab.txt",sep="\t")
    samTab
    df=newCountDataSetFromHTSeqCount(samTab)
    df

    cds<-estimateSizeFactors(df)
    sizeFactors(cds)
    head(counts(cds,normalized=TRUE))

    cds=estimateDispersions(cds)
    head(cds)
    str(fitInfo(cds))
    head(fData(cds))

    res=nbinomTest(cds,"Nor","Can")
    head(res)
    id baseMean baseMeanA baseMeanB foldChange log2FoldChange pval padj
    A1 138.33103 129.97950 146.68255 1.1285052 0.17441313 0.9057131 1


    resSig = res[res$padj < 0.1, ]
    head(resSig[order(resSig$pval), ])
    id baseMean baseMeanA baseMeanB foldChange log2FoldChange pval padj
    NA <NA> NA NA NA NA NA NA NA
    NA.1 <NA> NA NA NA NA NA NA NA
    NA.2 <NA> NA NA NA NA NA NA NA
    NA.3 <NA> NA NA NA NA NA NA NA
    NA.4 <NA> NA NA NA NA NA NA NA
    NA.5 <NA> NA NA NA NA NA NA NA

    I don't understand why the padj is 1. I suppoose that is the reason for the NA's. Is there a way to view the annotations while in R? Any suggestions? Please assist.

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