Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • lagrace
    Junior Member
    • May 2017
    • 4

    #1

    NA's in data (using TopHat-HTSeq-DESeq)

    Hello all,

    I have some data that I have retrieved from GEO. I am using the TopHat-HTSeq-DESeq pipeline to analyze this data. Within linux, I am using R and ran into an issue with NA's in the data. I am trying to determine why there are so many NA's. I was told to view the annotations. Is that possible in R? If so, how?
    Not sure if there is some other reason. Below are the codes used:

    samTab=read.table(file="samTab.txt",sep="\t")
    samTab
    df=newCountDataSetFromHTSeqCount(samTab)
    df

    cds<-estimateSizeFactors(df)
    sizeFactors(cds)
    head(counts(cds,normalized=TRUE))

    cds=estimateDispersions(cds)
    head(cds)
    str(fitInfo(cds))
    head(fData(cds))

    res=nbinomTest(cds,"Nor","Can")
    head(res)
    id baseMean baseMeanA baseMeanB foldChange log2FoldChange pval padj
    A1 138.33103 129.97950 146.68255 1.1285052 0.17441313 0.9057131 1


    resSig = res[res$padj < 0.1, ]
    head(resSig[order(resSig$pval), ])
    id baseMean baseMeanA baseMeanB foldChange log2FoldChange pval padj
    NA <NA> NA NA NA NA NA NA NA
    NA.1 <NA> NA NA NA NA NA NA NA
    NA.2 <NA> NA NA NA NA NA NA NA
    NA.3 <NA> NA NA NA NA NA NA NA
    NA.4 <NA> NA NA NA NA NA NA NA
    NA.5 <NA> NA NA NA NA NA NA NA

    I don't understand why the padj is 1. I suppoose that is the reason for the NA's. Is there a way to view the annotations while in R? Any suggestions? Please assist.

Latest Articles

Collapse

  • kinpict.com
    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by kinpict.com
    The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.
    Today, 06:37 PM
  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
    by SEQadmin2





    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
    10-30-2026, 06:46 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 11-12-2026, 07:59 AM
0 responses
27 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-05-2026, 09:25 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 11-03-2026, 11:54 AM
2 responses
56 views
0 reactions
Last Post kinpict.com  
Started by SEQadmin2, 10-28-2026, 09:57 AM
0 responses
175 views
0 reactions
Last Post SEQadmin2  
Working...