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  • repinementer
    Member
    • Dec 2009
    • 80

    #1

    Tophat mean inner distance error for SOLID data

    Sorry I solved the above question.
    Now I'm unable to detect bowtie, the same old error.
    I learnt setting up path for tophat and bowtie previously and successfully installed and worked with them for few months.
    Now I installed new updated versions and did the same set up. This time bow tie is not detecting.
    Any suggestions.

    Code:
    tophat -r 20 test_ref reads_1.fq reads_2.fq
    
    [Fri Oct 29 11:33:47 2010] Beginning TopHat run (v1.1.2)
    -----------------------------------------------
    [Fri Oct 29 11:33:47 2010] Preparing output location ./tophat_out/
    [Fri Oct 29 11:33:48 2010] Checking for Bowtie index files
    [Fri Oct 29 11:33:48 2010] Checking for reference FASTA file
    [Fri Oct 29 11:33:48 2010] Checking for Bowtie
    	Bowtie version:			 0.12.7.0
    [Fri Oct 29 11:33:48 2010] Checking for Samtools
    	[FAILED]
    Error: bowtie not found on this system
    bashrc profile
    Code:
    cat .bashrc 
    # .bashrc
    
    # Source global definitions
    if [ -f /etc/bashrc ]; then
    	. /etc/bashrc
    fi
    #export PATH=$PATH:/home/choijk/Gireesh/tophat-1.0.13/bin 
    #export PATH=$PATH:/home/choijk/Gireesh/bowtie-0.11.3 
    export PATH=$PATH:/home/choijk/Gireesh/sofware/bowtie-0.12.7
    export PATH=$PATH:/home/choijk/Gireesh/sofware/tophat-1.1.2/bin 
    # User specific aliases and functions
    Versions checked
    $ bowtie-build --version
    bowtie-build version 0.12.7
    64-bit
    Built on sycamore.umiacs.umd.edu
    Tue Sep 7 17:19:06 EDT 2010
    #####################
    $ tophat -v
    TopHat v1.1.2

    Any suggestions?
    Last edited by repinementer; 10-28-2010, 07:46 PM. Reason: solved question and added another

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