I have a number of gene sequences obtained using illumina sequencing and they are in fasta format, where nucleotides with a score of <20 have been replaced with X. I am going to filter my sequences by removing sequences that have 50% X's however would simply removing any X's in each read be a better method?
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By removing any X's in each read, do you mean for example ACGTXXACGT -> ACGTACGT? That would be a bad idea since you'd be deleting two (poor quality) bases. -
Hi Maubp,
Yes thats what I mean I wasnt too sure if it would be a bad idea or not but you have put my mind at ease. Thank you for the reply!Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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