Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • clarissaboschi
    Member
    • Apr 2010
    • 63

    #1

    How to output 5 top hits using standalone blast

    I am using blast, and I would like to filter my results, not based on the best hit, but the top 5 hits.

    I do not want to use the option "-max_target_seqs" because it does not result in the correct number, and also it was reported some issues with this option.

    Any suggestions?
    My output is: -outfmt '7 qseqid sseqid pident evalue bitscore stitle length'

    thanks
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Adding "-num_alignments 5" will work.
    Last edited by GenoMax; 09-08-2017, 09:21 AM.

    Comment

    • clarissaboschi
      Member
      • Apr 2010
      • 63

      #3
      GenoMax, I tried to use -num_alignments 5 and did not work...I got many hits.

      Comment

      • SylvainL
        Senior Member
        • Feb 2012
        • 180

        #4
        Originally posted by clarissaboschi View Post
        GenoMax, I tried to use -num_alignments 5 and did not work...I got many hits.
        Are you sure all these hits do not have the same score?

        Comment

        • GenoMax
          Senior Member
          • Feb 2008
          • 7142

          #5
          You are probably getting more than one HSP per "accession hit". If you strictly want to limit that to one HSP per accession then you could try adding "-max_hsp 1". Keep in mind that adding these restrictions may cause you to miss some important hits.

          Comment

          Latest Articles

          Collapse

          • SEQadmin2
            Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
            by SEQadmin2



            Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

            Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
            ...
            Today, 09:55 AM
          • SEQadmin2
            New Genomics Technologies Take Aim at Long-Standing Limits
            by SEQadmin2


            Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

            We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
            ...
            09-28-2026, 10:25 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by SEQadmin2, Today, 05:39 AM
          0 responses
          10 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, Yesterday, 09:56 AM
          0 responses
          12 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 10-09-2026, 11:14 AM
          0 responses
          26 views
          0 reactions
          Last Post SEQadmin2  
          Started by SEQadmin2, 09-29-2026, 09:51 AM
          0 responses
          77 views
          0 reactions
          Last Post SEQadmin2  
          Working...