I am looking for a FASTA file and GTF file that I can use to align my RNA seq (Hiseq) data and then anotate differential expressed genes. I looked at Xebase database, Ensembel but could not find these files. Can someone point me to a DB where I can find X tropicalis FASTA and GTF/ Gff files.
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Ensembl download page
Species table in the middle of page. Type 'Xenopus' in the Filter box (right side of table header). There are links to download whole genome FASTA, cDNA FASTA, CDS FASTA, etc as well as GTF/GFF3 annotation files.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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