Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • zee
    NGS specialist
    • Apr 2008
    • 249

    #1

    Identification of non-coding RNAs from RNASeq data

    I am interested in finding noncoding RNAs, particularly small RNAs from Illumina or SOLiD sequencing data prepared with RNASeq protocol.
    Apart from intersecting my mapped reads with mirBASE or other annotated small RNAs, what could I use for detecting noncoding RNAs from from RNASeq data?
    I know that we could just do a small RNA prep but I'm sure there are people who have attempted this sort of approach before to maximize what they get out of their RNASeq.
  • NicoBxl
    not just another member
    • Aug 2010
    • 264

    #2
    miRDeep or miRTrap are good programs

    1. Map your reads to a reference genome (discard the reads who not )
    2. Map your reads to the miR from mirbase ( be aware of the isomirs )
    3. With the rest of your reads, use miRDeep or miRTrap

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    18 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    36 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...