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This is my first RNA-seq run, and I have been searching online, to no avail, to find a reason for the sharp peaks I see in the k-mer plot in FastQC.
I generated mRNA libraries with the KAPA mRNA HyperPrep kit, using oligo-dT beads for mRNA enrichment, and random priming for first strand synthesis. I ran these libraries on an Illumina HiSeq 3000 with 76bp paired-end sequencing.
I attached one forward and one reverse read from the same sample. Both show sharp peaks in the k-mer content plot. Does anyone know what these could be? I'd really appreciate some advice as to what they are and how I can deal with them.
I generated mRNA libraries with the KAPA mRNA HyperPrep kit, using oligo-dT beads for mRNA enrichment, and random priming for first strand synthesis. I ran these libraries on an Illumina HiSeq 3000 with 76bp paired-end sequencing.
I attached one forward and one reverse read from the same sample. Both show sharp peaks in the k-mer content plot. Does anyone know what these could be? I'd really appreciate some advice as to what they are and how I can deal with them.