after samtools sort -n, the bam header still has @HD SO:unsorted instead of @HD SO:queryname. Is this a bug?
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telos,
The author addresses that question in this this thread, specifically in point number 1 under "known issues". -
Thanks for pointing that out, I'd missed that. But to be clear, the issue I'm raising is not point number 1 ("Samtools does not write header "SO" tag"), but rather that Samtools doesn't change an existing "SO" tag after sorting, which results in an incorrect value for the the tag. I hope the author finds the time to correct this.Comment
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I believe the author was referring to both writing AND updating the SO tag with his statement.Comment
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The part about this topic feels surprisingly grounded, and the details on the page is the bit that made me slow down. I’d keep <a href="https://kinpict.com/">ai family photo generator</a> bookmarked for later.Today, 06:37 PM -
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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