I noticed at the qiime2 forum someone else asked the same question I have - How do these 2 compare (or perhaps contrast would be better focus?) for output other than faster speed for Deblur? The forum suggested the person ask here at seqanswers, but it doesn't seem like they followed up. Can someone tell me anything about this that would be helpful as I move forward analyzing 16s V3 region amplicons. Thanks!
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Hi urchin i just finished comparing DADA2 and deblur and have concluded that DADA2 is the correct decision. We use v3v4 and DADA2 gives 3 to 4 times as much high-quality data in return as deblur.
My thought is deblur is for very short sequences because it was close to DADA2 when i chopped to 100bp like in the deblur paper : but we use the whole v3v4 for better assignment. Perhaps v3 is very short? Otherwise i would recommmend DADA2.
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