how does sequencing accuricy come out?

[holywoool]

Hi,
As i know,the SOLiD system is the only next-generation platform to demonstrate overrall accuricy greater than 99.94%.Then how does this number come out?whether it has the following formular:
accuracy_rate=100%-Total_mismatch/Total_reads*read_lenth

[Torst]

Hi,
As i know,the SOLiD system is the only next-generation platform to demonstrate overrall accuricy greater than 99.94%.Then how does this number come out?whether it has the following formular:
accuracy_rate=100%-Total_mismatch/Total_reads*read_lenth

Illumina also claim "99.5% mappable reads". The problem with this is that they calculate this AFTER they have thrown away lots of reads, which they know won't be mappable...

[krobison]

Hi,
As i know,the SOLiD system is the only next-generation platform to demonstrate overrall accuricy greater than 99.94%.Then how does this number come out?whether it has the following formular:
accuracy_rate=100%-Total_mismatch/Total_reads*read_lenth

You forgot the critical parens in typing this

accuracy_rate=100%-Total_mismatch/(Total_reads*read_length)