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Hi,
I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and also shearing during library prep stage? How to validate the existence of a large insert such as 3Mb or 17 Mb as it cannot be confirmed even by a high fidelity Taq (max limit of Genomic DNA pcr is 1-2kb for taq and upto 30kb for hf Taq)?
Regards
I am visualizing alignments using IGV. I want to ask what do you mean by Insert? Is insert size a large unsequenced segment sitting right in between the two reads which escapes sequencing and also shearing during library prep stage? How to validate the existence of a large insert such as 3Mb or 17 Mb as it cannot be confirmed even by a high fidelity Taq (max limit of Genomic DNA pcr is 1-2kb for taq and upto 30kb for hf Taq)?
Regards