Can someone provide a detailed explanation, what the bowtie2 parameter "--dpad" does? The explanation given in the manual is as follows: "--dpad <int> include <int> extra ref chars on sides of DP table (15)".
I would interprete it like this:
1) A sequence string of length <int> is attached to every 5' and 3' end of a read after seeding and global alignment.
2) Attached sequences are used to solve mapping issues at readends (e.g. deletions present at readends that have been falsley softclipped in previous steps).
!!!But I am really not confident with this!!!
I am asking because I realised that increasing dpad to an integer of 100, solves issues I have to face with large deletions in a merged 350bp Illumina amplicon dataset. I mean solving this issue is fine, but I do not have a clue what the possible drawbacks of this setting might be. Furthermore, as I do not exactly understand the parameter and its behaviour, I do not know how to test for possible side-effects.
Here are my option settings:
bowtie2 --rdg 5,1 --rfg 5,1 --dpad 100 --score-min L,0,-0.75 --very-sensitive
I would interprete it like this:
1) A sequence string of length <int> is attached to every 5' and 3' end of a read after seeding and global alignment.
2) Attached sequences are used to solve mapping issues at readends (e.g. deletions present at readends that have been falsley softclipped in previous steps).
!!!But I am really not confident with this!!!
I am asking because I realised that increasing dpad to an integer of 100, solves issues I have to face with large deletions in a merged 350bp Illumina amplicon dataset. I mean solving this issue is fine, but I do not have a clue what the possible drawbacks of this setting might be. Furthermore, as I do not exactly understand the parameter and its behaviour, I do not know how to test for possible side-effects.
Here are my option settings:
bowtie2 --rdg 5,1 --rfg 5,1 --dpad 100 --score-min L,0,-0.75 --very-sensitive
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