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Hi there, I'm new to the forum and relatively new to the field.
I have a transcriptome in a almost 200MB fasta. I was running TGICL with various CAP3 settings for removing chimaeras and redundant sequences in another system when a "fairly" catastrophic failure occurred and the whole Operative System had to be reinstalled.
I was getting around 15000 sequences (contigs and singlets) but, after the reinstallation, with the same transcriptome, I'm getting 205 contigs and 10 singlets although the script runs without any detectable error.
Has anybody had this problem before or knows what could be happening?
The program was installed with:
tar xvf TGICL-2.1.tar.gz
cd TGICL-2.1/
perl Build.PL
perl Build
sudo perl Build install
I have a transcriptome in a almost 200MB fasta. I was running TGICL with various CAP3 settings for removing chimaeras and redundant sequences in another system when a "fairly" catastrophic failure occurred and the whole Operative System had to be reinstalled.
I was getting around 15000 sequences (contigs and singlets) but, after the reinstallation, with the same transcriptome, I'm getting 205 contigs and 10 singlets although the script runs without any detectable error.
Has anybody had this problem before or knows what could be happening?
The program was installed with:
tar xvf TGICL-2.1.tar.gz
cd TGICL-2.1/
perl Build.PL
perl Build
sudo perl Build install