Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • agc
    Member
    • May 2010
    • 26

    #1

    Tablet not showing pileup indels

    Hi,

    I have a pileup file with the following line:
    Code:
    chr4	103518793	*	*/+TA	20	20	33	6	*	+TA	5	1	0	0	0
    I understand this implies that there is an indel at position 103518793 on chr4. However, upon opening the bam file in tablet and checking said location, I see 6 reads, none of which support an indel.

    Does anyone know what might cause this?

    Thanks.
  • gaffa
    Member
    • Oct 2010
    • 82

    #2
    Tablet does not display insertions by putting a gap in the reference sequence, but by putting an annotation in a feature track running alongside the alignment. This is the "CIGAR-I" track (if you don't have it by default you should be able to add it in "Select Tracks" in the "Features" tab) - an insertion is marked by a big "I" in this track, hoover the "I" and it shows you what read(s) have the insertion.

    It works but it's a bit awkward in my opinion. Having an option for displaying gaps in the reference sequence (like in e.g. samtools tview) would be pretty neat.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM
    • SEQadmin2
      New Genomics Technologies Take Aim at Long-Standing Limits
      by SEQadmin2


      Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

      We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
      ...
      09-28-2026, 10:25 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Yesterday, 11:13 AM
    0 responses
    15 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 10:48 AM
    0 responses
    13 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-15-2026, 10:52 AM
    0 responses
    35 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-14-2026, 05:39 AM
    0 responses
    42 views
    0 reactions
    Last Post SEQadmin2  
    Working...