Hi everyone,
I have questions on the rebuilt isofroms/transcripts in the different steps of Cufflinks tool. I found that the rebuilt transcripts recovered by cufflinks and the transcripts in isoform_exp.diff created by cuffdiff are different. My questions are:
1)Cuffdiff re-assign the reads, rebuild the transcripts, and re-estimate the expression level?
2)How can I track the structure of the transcripts in isoform_exp.diff?
Thank you.
I have questions on the rebuilt isofroms/transcripts in the different steps of Cufflinks tool. I found that the rebuilt transcripts recovered by cufflinks and the transcripts in isoform_exp.diff created by cuffdiff are different. My questions are:
1)Cuffdiff re-assign the reads, rebuild the transcripts, and re-estimate the expression level?
2)How can I track the structure of the transcripts in isoform_exp.diff?
Thank you.
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