Tweet
Hello everybody,
I can see that my 1Mio 454 reads are full of insertions/deletions in homopolymer regions, and of course this disrupts my ORFs in later stages (annotation step). Fortunately, I have ca 60mio Illumina reads that I can use to correct these errors. Both dataset are cDNA.
Has anyone a preferred method to correct short sequencing error indels? If tractable, I would prefer correcting my reads rather than correcting my draft assembly obtained from those reads. I was going to use ssaha (or segemehl, qpalma, other?) to map the illumina reads on my 454 reads, and then extract a consensus by parsing the pileup file generated with samtools
I have seen a few published, more advanced, methods available but I*do not now if one of them performs better (e.g. iCorn). Do you?
Thanks a lot!
Yvan
I can see that my 1Mio 454 reads are full of insertions/deletions in homopolymer regions, and of course this disrupts my ORFs in later stages (annotation step). Fortunately, I have ca 60mio Illumina reads that I can use to correct these errors. Both dataset are cDNA.
Has anyone a preferred method to correct short sequencing error indels? If tractable, I would prefer correcting my reads rather than correcting my draft assembly obtained from those reads. I was going to use ssaha (or segemehl, qpalma, other?) to map the illumina reads on my 454 reads, and then extract a consensus by parsing the pileup file generated with samtools
I have seen a few published, more advanced, methods available but I*do not now if one of them performs better (e.g. iCorn). Do you?
Thanks a lot!
Yvan
Comment