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  • seqlad
    Junior Member
    • Mar 2018
    • 2
    #1

    Runing MOABS on Bismark BAM files

    Hello,

    I have BAM files generated with Bismark 0.19.0. I wish to detect differentially methylated Cytosines (CpG or non-CpG context). I have tried to run the MOABS mcall tool on those files (As suggested in the MOABS documentation) I get an error message:

    program is parsing file according to BAM foramt
    XR:Z or ZR:Z:, or ZS:Z: field not found, please enable -R option in bsmapXR:Z or ZR:Z:, or ZS:Z: field not found, please enable -R option in bsmap

    Has anyone ever tried to run MOABS on bismark BAM files ?
    Any idea as to how solve that problem would be greatly appreciated!
    Also if you know some other tool that I could use to find DMCs from Bismark file please let me know!

    Thanks
    Tags: bismark, dmc, methylome, moabs
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