Hi all,
I have a question on calculating the overall depth of coverage for exome or targeted panel sequencing samples.
As a simple example. If I have paired-end reads that overlap each other. A particular base-pair, would have been sequenced twice, once by the forward read and once by the reverse read. When calculating depth at the base, would you consider this base as having a 1X coverage or a 2X coverage. Essentially treating each individual read of a paired-end as a separate event, or considering them as as combined single event at the base?
Thanks
I have a question on calculating the overall depth of coverage for exome or targeted panel sequencing samples.
As a simple example. If I have paired-end reads that overlap each other. A particular base-pair, would have been sequenced twice, once by the forward read and once by the reverse read. When calculating depth at the base, would you consider this base as having a 1X coverage or a 2X coverage. Essentially treating each individual read of a paired-end as a separate event, or considering them as as combined single event at the base?
Thanks
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