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  • duna
    Junior Member
    • Apr 2018
    • 1

    #1

    problem with signalP not working

    Hello everybody, and thank you to have accepted me in the forum!

    I am trying to make signalP 4.1 working on iOS 10.13.3 since days and I hope that someone can help me

    I downloaded the package for the darwin system and followed the installation instructions. But when I made the test

    > ./signalp -t euk -f short test/euk10.fsa > euk10.fsa.short_out

    I obtained a different output then expected

    ----
    # SignalP-4.1 euk predictions
    # Measure Position Value Cutoff signal peptide?
    max. C 1 0.000
    max. Y 1 0.000
    max. S 1 0.000
    mean S 1-0 0.000
    D 1-0 0.000 0.450 NO
    Name= SP='NO' D=0.000 D-cutoff=0.450 Networks=SignalP-noTM
    ---

    and the same if I test a home made file of amminoacid contigs like this

    ---
    >a
    QKMKFKFVYILQYNYNCTSLQKNDKIALFSLPPTLSANGGVSLIKRGWHEIPEIMCCLGMIGISTVFLGISIVRYDADLNHQYKFQYTVVRDTDVRDEWVKKKMYN
    >b
    LLLITRVHHSSPTVFSTSYARYYATVLGCLVVHFLLHPFVPHISVSNHCVLKFVLVIQVCVVADNTNSQEHS
    >c
    TRVFSRIELAGERLNCPAAFPLHVSTSTVQTVQKAERRTAPPPLRLATTLGSRPAGHQGQHYSPLPHP
    >d
    KIPSSCRSVESCICCIEGVLAWKVCSPRANAEIETERESETESETEKETDRQTDRQTDRQTDRETKTDRKTKTD
    >e
    KGRGLAGYEPAYSHVLNWQANVLTVLPLSPFMSVQVPSRQYKKPKDVLPLRPSDLPQPWAADQPDIKVSTTVPYLIPKDGQDDQVKQTLTLATFVERYPLAVEAWSHVYVVLRVYWRGRCALHGQMPK
    >f
    ASGKRRPLSQPSGDPSSQMVHLNSQSFPNTPYRNEMKVTLLGRIGKIGMLSGIWQTGGIGQWPNA
    >g
    TAGSQVYPEVYLPSGKCPEIRCMRLKRGRYHMNLWGNFTGPT
    >h
    LAMKVSSVLTTGEWPYRFIPDLSRDSLAMKVSSGLTTGEWPYRFIPDLSRDSLAMKVSSALTTGEWPYRFIPDLSRDSLAMKVSSGLTTGEWPYRFIPDLSRDSLAMKVSS
    ----

    with this command

    ./signalp -t euk -f short /path/prova2.fasta > /path/output.txt

    I get this output file

    ----
    # SignalP-4.1 euk predictions
    # name Cmax pos Ymax pos Smax pos Smean D ? Dmaxcut Networks-used
    0.000 1 0.000 1 0.000 1 0.000 0.000 N 0.450 SignalP-noTM
    ----

    but no error message appeared... someone know what can be the problem?

    Thank you for any help!

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