I am running cufflinks on single and mate-paired ABI SOLiD results from tophat. The cufflinks manual says library type for standard SOLiD is fr-secondstrand. I thought pair-end solid data has both reads pointing the same direction. Shouldn't I use ff-secondstrand?
-
The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...09-01-2026, 05:41 AM