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  • ljhwahaha
    Junior Member
    • Oct 2010
    • 3

    #1

    What is the length limitation of sequences for newbler?

    I have a wide range length of sequences which were intended to assemble using a overlap assembler, such as newbler. But i don't know the length limitation of newbler, most of my sequences are longer than 400bp, some of them are longer than 10K bp.
    Please help me if you know that, thanks.
    If you have any suggestion, please tell me.
  • flxlex
    Moderator
    • Nov 2008
    • 412

    #2
    As far as I know the limit is 2kb

    Comment

    • ljhwahaha
      Junior Member
      • Oct 2010
      • 3

      #3
      Originally posted by flxlex View Post
      As far as I know the limit is 2kb
      Could I modify it?

      Comment

      • flxlex
        Moderator
        • Nov 2008
        • 412

        #4
        No. :-( One thing to try is to generate 2kb parts (with overlap) from the reads that are too long. Never done it, though...

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