Hi,
After using a FastQC I noticed that I have red flags in Per base sequence content and in Per base content.
I used Trimmomatic for solving these problems, but it solve problem only for per base N content, but not for per base sequence content. Moreover, after using Trimmomatic I have yellow flag in sequence length distribution.
Could you help me how to resolve these problems?
Thank you in advance.
After using a FastQC I noticed that I have red flags in Per base sequence content and in Per base content.
I used Trimmomatic for solving these problems, but it solve problem only for per base N content, but not for per base sequence content. Moreover, after using Trimmomatic I have yellow flag in sequence length distribution.
Could you help me how to resolve these problems?
Thank you in advance.