I want to find out the mode of action of the novel compounds.
I have generated the mutants of mycobacterium using high concentrations of these compounds and got the data for whole genome sequencing. I would like to compare this data with that of the wild type and see if there are any point mutations or SNPs. If they are any mutations, on what gene or genes they are associated with.
Thanks
Kind Regards
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM