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  • Ling1
    Junior Member
    • Dec 2010
    • 1

    #1

    samtools mpileup

    Hi everyone,

    When I try mpileup in samtools-0.1.12.
    Code:
    samtools-0.1.12/samtools mpileup -C50 -gf ref.fasta file.bam  > file.bcf
    There is segfault:
    [mpileup] 1 samples in 1 input files
    <mpileup> Set max per-sample depth to 8000
    Segmentation fault

    Also, I tried
    Code:
    samtools-0.1.12/samtools mpileup -ugf file.fasta file.bam | samtools-0.1.12/bcftools/bcftools view -bvcg - > var.raw.bcf
    
    samtools-0.1.12/bcftools/bcftools view var.raw.bcf | samtools-0.1.12/bcftools/vcfutils.pl varFilter -D100 > var.flt.vcf
    
    awk '{print $1}' var.flt.vcf | sort | uniq -c
    The output after awk was:
    12111 10
    1640 11
    1 #CHROM
    1 ##fileformat=VCFv4.0

    Can anyone give me some hints on what the problem is? Thank you!
  • yina
    Junior Member
    • Jul 2009
    • 6

    #2
    Hi I have the same problem, did you found the solution?? any hint would help

    Comment

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