Header Leaderboard Ad

Collapse

What is the process after mapping ?

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • What is the process after mapping ?

    Hi guys,

    I have a raw data, which is rna-seq paired end. I have downloaded genome reference from ncbi. Then, I have mapped rna seq to genome reference through tophat2. I had some results like accepted.hits.bam. After this process, I used the cufflinks for assembly. Finally, I have a transcripts.gtf file. Firstly, I do not know how to interprate these files. Secondly, After all this process, What i should do ? I mean, What options do i have ?
    Well, Should i use artemis or igv ? Actually i dont really know.

    Thanks in advance,
    your respectfully,

  • #2
    What is the question you are trying to answer by doing this mapping?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      A Brief Overview and Common Challenges in Single-cell Sequencing Analysis
      by seqadmin


      ​​​​​​The introduction of single-cell sequencing has advanced the ability to study cell-to-cell heterogeneity. Its use has improved our understanding of somatic mutations1, cell lineages2, cellular diversity and regulation3, and development in multicellular organisms4. Single-cell sequencing encompasses hundreds of techniques with different approaches to studying the genomes, transcriptomes, epigenomes, and other omics of individual cells. The analysis of single-cell sequencing data i...

      01-24-2023, 01:19 PM
    • seqadmin
      Introduction to Single-Cell Sequencing
      by seqadmin
      Single-cell sequencing is a technique used to investigate the genome, transcriptome, epigenome, and other omics of individual cells using high-throughput sequencing. This technology has provided many scientific breakthroughs and continues to be applied across many fields, including microbiology, oncology, immunology, neurobiology, precision medicine, and stem cell research.

      The advancement of single-cell sequencing began in 2009 when Tang et al. investigated the single-cell transcriptomes
      ...
      01-09-2023, 03:10 PM

    ad_right_rmr

    Collapse
    Working...
    X