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  • how to get SNPs matrix for population genetic ananlysis

    Dear seqanswers,

    I am new to genomics and bioinformatics. In my current study, we have
    sequenced the genomes of tens of accessions of a plant, using Illumina
    next generation sequencer. The short reads of a specific accession
    have been aligned to the reference. The SNPs and shor indels have been
    predicted for a specific accession genome to the reference. We have gotten the separate files for SNPs like the following format (in text file, the column
    names were listed to each accession, the accession name will not change for a specific accession):

    <accession names> <chromosome><position><reference base><cons
    base><quality><support><concordance><avg_hits>


    But usually, we need to align all the accessions in the following
    format for classical population genetic analysis:

    <accessions><SNP_1><SNP_2><SNP_3><SNP_...>
    accession_1, a,t,g,,,
    accession_2, a,t,c,,,
    accession_3, t,a,c,,,
    accession_,,,,,,,,,,,,,

    I need to get helps, suggestions on how to do this format conversion,
    or if there are any alternative choices for me, by using R and
    bioconductor? If it need database operations, and how to do that?

    Thanks in advance.

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