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NORMALIZATION Illumina DESeq

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  • NORMALIZATION Illumina DESeq

    Dear colleagues

    In my group, we are trying to give an answer to our results, but the first problem is normalization. We have used DESeq script for R and it´s great¡¡¡ but we need to compare 3 independent samples without replicates, anybody knows how could we resolve it?

    Thanks a lot¡¡

  • #2
    Dear Htfriedelff

    have a look at section 6 of the DESeq vignette, "Working without any replicates".
    Replicates do, however, tend to be important for biologically meaningful experiments, the DESeq software will not work any magic here.

    Wolfgang
    Wolfgang Huber
    EMBL

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