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  • Stephanbio
    Junior Member
    • Dec 2010
    • 5

    #1

    Convert chromosomal position to gene sequence position

    I have a list of mutations with their chromosomal locations like
    chr1:6266188-6266188
    chr22:5443534-5443534 .....

    I know how to fetch a list of genes with ucsc browser and/or galaxy that intersects with my mutations. and their accompanying DNA sequence

    But in order to know wich nucleotide in the gene sequence is mutated I have to know the position in the gene.
    Is there a way to convert the chromosomal position to nucleotide position in a gene. so that I can easy invistigate what kind of mutation it is.
    dream result would be:
    gene1 blabla lenght: 1...789 your mutation is in place 88
    or actaXacta
    or actaNacta
  • ZhengXia
    Member
    • Feb 2009
    • 10

    #2
    If you fetch gene region from annotation database such as UCSC knowngene.txt, you can easily do it.
    Originally posted by Stephanbio View Post
    I have a list of mutations with their chromosomal locations like
    chr1:6266188-6266188
    chr22:5443534-5443534 .....

    I know how to fetch a list of genes with ucsc browser and/or galaxy that intersects with my mutations. and their accompanying DNA sequence

    But in order to know wich nucleotide in the gene sequence is mutated I have to know the position in the gene.
    Is there a way to convert the chromosomal position to nucleotide position in a gene. so that I can easy invistigate what kind of mutation it is.
    dream result would be:
    gene1 blabla lenght: 1...789 your mutation is in place 88
    or actaXacta
    or actaNacta

    Comment

    • Stephanbio
      Junior Member
      • Dec 2010
      • 5

      #3
      Now that's exactly my question! If it's so easy, can you explain further
      Last edited by Stephanbio; 12-21-2010, 01:05 AM.

      Comment

      • Jonathan
        Member
        • Jun 2009
        • 36

        #4
        http://seqanswers.com/wiki/ANNOVAR

        is what your are looking for.

        Comment

        • Joann
          Senior Member
          • Oct 2008
          • 230

          #5
          more open research

          I suggest ANNOVAR for publication in the new journal Open Research Computation--recently introduced elsewhere on this site.

          Comment

          • Stephanbio
            Junior Member
            • Dec 2010
            • 5

            #6
            Thanx , I think ANNOVAR is exactly what i need
            I'm playing with it right now.

            Comment

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