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bbduk useshortkmers - no documentation
I am following an Illumina recommended pipeline which includes the bbduk option "useshortkmers=t". This option is not referenced in the bbduk docs, or the -h. Google only finds examples of it being invoked - with no explanations other than it conflicts with maskmiddle. I have tried with and without the option which appears to make no difference. I am curious to know if it is redundant and if not, what it does. Does anybody know? Thanks!
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.Yesterday, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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