I am following an Illumina recommended pipeline which includes the bbduk option "useshortkmers=t". This option is not referenced in the bbduk docs, or the -h. Google only finds examples of it being invoked - with no explanations other than it conflicts with maskmiddle. I have tried with and without the option which appears to make no difference. I am curious to know if it is redundant and if not, what it does. Does anybody know? Thanks!
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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