We have 50bp reads and want to map with bowtie with 6mm. However it seems that only 3 mm are allowed at max. I have tried to look on net and many people suggested to use -e option..I am not sure what would be the best parameters to map SOLiD 50bp reads with 6mm ? Can anyone help on this?
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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