Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • the_august
    Junior Member
    • Jan 2013
    • 6

    #1

    Looking for the sequence of a particular transcript from sequencing data

    First, let me tell you that I'm very new to the field of NGS and not much familiar with the set of tools commonly used for NGS data processing. However, I'm not new to Bioinformatics, and have quite some experience in Linux, Python, Perl , R etc.

    I've to find out the sequence of a particular transcript from a set of mouse fastq files. I've extracted the sequence reads for this particular sequence using bbduk.sh of the bbmap package using the following command:

    Code:
    bbduk.sh  in=/me/clumpedSRA.fq.gz ref=/me/known_transcript.fasta k=13 outm=/me/knownT_reads_only.fastq
    Can somebody tell me how to get the sequence of the transcript from this reads of the sequence of interest? Can any other tools from 'bbmap' be used?

    Besides, I've access to Tophat and Trinity on a high performance cluster.

    Thanks in advance

Latest Articles

Collapse

  • SEQadmin2
    Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
    by SEQadmin2



    Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

    Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
    ...
    10-14-2026, 09:55 AM
  • SEQadmin2
    New Genomics Technologies Take Aim at Long-Standing Limits
    by SEQadmin2


    Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.

    We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing
    ...
    09-28-2026, 10:25 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 10-22-2026, 11:13 AM
0 responses
35 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-22-2026, 10:48 AM
0 responses
25 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-15-2026, 10:52 AM
0 responses
41 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 10-14-2026, 05:39 AM
0 responses
45 views
0 reactions
Last Post SEQadmin2  
Working...