NGS data analysis

[wanadou]

I am beginner in the field of Bioinformatics
I received HiSeq illuminina 100 pb PE sequencing clean data as a fastq file
what are the steps to successfully assemble this genome of 400 MB?
It is necessary to join the multiple files fastq in one file to make the assembly?
thx for your help

[GenoMax]

You can take a look at this WikiBoooks entry on NGS data genome assembly to get an indea of what would be involved.

If you have a close/related reference genome available in the databases you could use that to do a reference assisted assembly as well.